Hoʻokaʻawale a me ka hoʻohana ʻana o ka pā hohonu hohonu
Ka hoʻokaʻawale ʻana o ka pā punawai hohonu:
1. E like me ka helu o nā puka, ʻo nā mea maʻamau he 96 mau papa puka a me 384 mau papa puka.
2. E like me ke ʻano o ka lua, hiki ke hoʻokaʻawale ʻia nā papa puka 96 i loko o ke ʻano o ka lua a me ke ʻano lua.384 punawai na lua huinaha.
3. E like me ke ʻano o ka lua lalo, aia ka nui o ka U a me ka V.
Ka hoʻokaʻawale ʻana o ka pā hohonu orifice:
He aha ke kumu o ka pā punawai hohonu?
① Nā laʻana mālama:
Hiki iā ia ke hoʻololi i ka 1.5ml centrifuge tube maʻamau e mālama i nā laʻana, a ua hoʻokomo pono ʻia i loko o ke kaʻina hana mālama, mālama i ka wahi, me ka nui o ka mālama ʻana, a hiki ke kū i ka friji o - 80 ℃.Kapa ʻia hoʻi he poloka mālama.
② Laʻana lapaʻau:
Hiki ke hoʻohana pū ʻia me nā pū hoʻokuʻu, kiʻekiʻe-throughput mīkini hana wai a me nā lako polokalamu e hoʻokō i nā hana kiʻekiʻe-throughput ma nā laʻana olaola, e like me ka ua o ka protein a me ka ʻili wai-wai.Hoʻomaikaʻi maikaʻi ʻia ka maikaʻi o ka hana ʻana o ka hāpana.Hiki i nā mea PP ke kū i ka wela kiʻekiʻe a me ka hoʻomaʻamaʻa sterilization kiʻekiʻe ma 121 ℃.
③ Hana laʻana:
Hoʻohana maʻamau ia i nā samplers ʻokoʻa like ʻole a hiki ke waiho pololei ʻia i loko o ke keʻena hāpana o ka sampler aunoa no ka injection.Ke hoʻohālikelike ʻia me ka ʻōmole hoʻohālike maʻamau, ʻaʻole hiki ke pāpālua wale i ka helu o nā laʻana i loko o ka lumi laʻana, akā ʻike pū kekahi i ka waiho ʻana.Ma hope o ka hana ʻana ma ka pā 96 hole, hiki ke hoʻopili pololei ʻia ka laʻana me ka ʻole o ka hana luhi.E kahakiʻi i ka hāpana i hope a i waho, e kau i ka hāpana, e uhi iā ia, e hoʻokomo i ka plug-in a hoʻomaʻemaʻe i ka ʻōmole.
He kūpono loa ka pā luawai hohonu o Labio no ka mālama ʻana i nā laʻana, ka nānā ʻana i ke kiʻekiʻe throughput screening (HTS) e koi ana i ka motherboard, cell and tissue culture, immunological analysis a me nā noi ʻē aʻe.He 96 a i ʻole 384 mau puka ka pā, e hāʻawi ana i ka mana 0.5mL, 1.2mL, 2.0mL a me 2.2mL.Hōʻailona ʻia nā puka ma nā ʻano alphanumeric maʻamau e hoʻomaʻamaʻa i ka hōʻailona hōʻailona a hiki ke hoʻopaʻa ʻia no ka mālama ʻana.He maʻalahi ka ʻimi notch i loko o ka robotic sampler a me ka ʻōnaehana hoʻokele wai.
ʻO nā pāpaʻi polypropylene (PP) ʻole sterile i hana ʻia me ka propylene maka kiʻekiʻe hiki ke hoʻopaʻa ʻia a kūʻē i nā ʻano kemika like ʻole, me ka phenol, chloroform a me DMS.Eia kekahi, hiki i ka papa PP ke pale i nā mahana haʻahaʻa e like me - 80 ° C / - 112 ° F, i mea kūpono no nā noi lumi anuanu a me ka mālama haʻahaʻa haʻahaʻa.E koho i ka papa polystyrene no ka loaʻa ʻana o ka mālamalama a i ʻole nā waiwai o luna.
Hiki ke hoʻohana pū ʻia nā papa ʻo Labio 96 hole a me 384 me ka silicone gasket a i ʻole ke kiʻi ʻoniʻoni hoʻopaʻa paʻa e hōʻemi i ka hoʻoheheʻe ʻana a me ka haumia.
Ka manawa hoʻouna: Nov-25-2022