ʻO ka hōʻiliʻili laʻana, mālama a me ka lawe ʻana i nā koi no nā hoʻokolohua maʻamau

ʻO ka hōʻiliʻili laʻana, mālama a me ka lawe ʻana i nā koi no nā hoʻokolohua maʻamau

1. ʻOhi a mālama ʻana i nā specimen pathological:

☛ ʻĀpana paʻa: Wehe i nā poloka kiko kūpono a mālama iā lākou i loko o ka hau hau;

☛Ka ʻāpana paraffin: Wehe i nā poloka kiko kūpono a mālama iā lākou i loko o 4% paraformaldehyde;

☛Nā paheʻe kelepona: Ua hoʻopaʻa ʻia nā paheʻe kelepona i 4% paraformaldehyde no 30 mau minuke, a laila pani ʻia me ka PBS a hoʻokomo ʻia i loko o ka PBS a mālama ʻia ma 4°C.

2. ʻOhi a mālama ʻana i nā ʻano molecular biology specimen:

☛Nā ʻiʻo hou: E ʻoki i ka mea hoʻohālike a mālama i loko o ka hau hau a i ʻole -80°C;

☛ Nā mea hoʻohālike paraffin: E kūʻai ma ka lumi wela;

☛Ke koko holoʻokoʻa: E lawe i ka nui kūpono o ke koko holoʻokoʻa a hoʻohui i ka EDTA a i ʻole heparin anticoagulation ohi koko;

☛Nā ʻano wai o ke kino: ʻo ka centrifugation kiʻekiʻe e hōʻiliʻili i ka sediment;

☛Nā hoʻohālike o ke kelepona: Hoʻopaʻa ʻia nā kelepona me TRizol a mālama ʻia i loko o ka wai hau a i ʻole -80°C pahu hau.

3. ʻOhi a mālama ʻana i nā mea hoʻokolohua protein:

☛Nā ʻiʻo hou: E ʻoki i ka mea hoʻohālike a mālama i loko o ka hau hau a i ʻole -80°C;

☛Ke koko holoʻokoʻa: E lawe i ka nui kūpono o ke koko holoʻokoʻa a hoʻohui i ka EDTA a i ʻole heparin anticoagulation ohi koko;

☛Nā helu kelepona: Hoʻopili piha ʻia nā cell me ka solution lysis cell a laila mālama ʻia i loko o ka wai hau a i ʻole -80°C pahu hau.

4. ʻOhi a mālama ʻana i nā mea hoʻokolohua ELISA, radioimmunoassay, a me biochemical hoʻokolohua.

☛Serum (plasma) laʻana: E lawe i ke koko holoʻokoʻa a hoʻohui i loko o kahi paipu procoagulation (anticoagulation tube), centrifuge ma 2500 rpm no kahi o 20 mau minuke, e hōʻiliʻili i ka supernatant, a mālama i loko o ka wai hau hau a i ʻole -80 ° C;

☛Urine laʻana: centrifuge i ka hāpana ma 2500 rpm no kahi o 20 mau minuke, a mālama i loko o ka wai hau a i ʻole -80°C pahu hau;e nānā i kēia ʻano no ka wai o ka ʻōpū a me ka ascites, ka wai cerebrospinal, a me ka wai holoi alveolar;

☛ Nā laʻana cell: I ka ʻike ʻana i nā ʻāpana huna, e centrifuge i nā laʻana ma 2500 rpm no kahi o 20 mau minuke a mālama iā lākou i loko o ka wai hau a i ʻole -80°C friji;i ka ʻike ʻana i nā ʻāpana intracellular, E hoʻoheheʻe i ka hoʻokuʻu ʻana o ke kelepona me ka PBS a hoʻokuʻu a hoʻoheheʻe pinepine ʻia e luku i nā ʻāpana a hoʻokuʻu i nā ʻāpana intracellular.Centrifuge ma 2500 rpm no kahi o 20 mau minuke a hōʻiliʻili i ka supernatant e like me luna;

☛ Nā laʻana kikokikona: Ma hope o ka ʻokiʻoki ʻana i nā mea hoʻohālike, e kaupaona iā lākou a hoʻokuʻu iā lākou i loko o ka nitrogen wai a i ʻole -80°C pahu hau no ka hoʻohana ʻana ma hope.

5. ʻO ka hōʻiliʻili huaʻōlelo Metabolomics:

☛Urine laʻana: Centrifuge i ka hāpana ma 2500 rpm no kahi o 20 mau minuke a mālama i loko o ka wai hau a i ʻole -80°C pahu hau;e nānā i kēia ʻano no ka wai o ka ʻōpū a me ka ascites, ka wai cerebrospinal, ka wai holoi alveolar, etc.;

☛Ma hope o ka ʻoki ʻana i ka laʻana o ka ʻiʻo, kaupaona a hoʻokuʻu ʻia i loko o ka hau hau a i ʻole -80 ° C no ka hoʻohana ʻana ma hope;