ʻO kaELISAʻO ka pāpaʻi kahi mea hana pono no ELISA, ka hoʻāʻo immunosorbent pili i ka enzyme.Nui nā kumu e pili ana i ka holomua o nā hoʻokolohua ELISA.ʻO ke koho ʻana i ka mea hana pono ka hana mua.ʻO ke koho ʻana i kahi microplate kūpono e kōkua i ka hoʻokolohua e kūleʻa.
ʻO ka mea o kaELISAʻO ka pāpaʻi maʻamau he polystyrene (PS), a ʻo ka polystyrene he paʻa kemika maikaʻi ʻole a hiki ke hoʻoheheʻe ʻia e nā ʻano mea hoʻoheheʻe kūlohelohe (e like me ka hydrocarbons aromatic, halogenated hydrocarbons, etc.), a hiki ke hoʻopau ʻia e nā ʻakika ikaika a me nā alkalis.ʻAʻole kūʻokoʻa i ka momona a maʻalahi hoʻi ka ʻulaʻula ma hope o ka ʻike ʻia ʻana i ke kukui UV.
He aha nā ʻanoELISAAia nā papa?
✦E koho ma ke kala
ʻO ka pā alohilohi:kūpono no ka quantitative a qualitative solid-phase immunoassays a me ka hoʻopaʻa ʻana;
Papa keʻokeʻo:kūpono no ka luminescence pono'ī a me ka chemiluminescence;
Papa ʻeleʻele:kūpono no nā immunoassay fluorescent a me nā hoʻopaʻa paʻa.
✦E koho ma ka hoʻopaʻa ikaika
Pāpā haʻahaʻa:Hoʻopaʻa paʻa i nā protein ma o nā paʻa hydrophobic ili.He kūpono ia ma ke ʻano he mea lawe paʻa paʻa no nā protein macromolecular me ke kaumaha molekala> 20kD.ʻO kona hiki ke hoʻopaʻa i ka protein he 200~300ng IgG/cm2.
Paʻa paʻa kiʻekiʻe:Ma hope o ka mālama ʻana i ka ʻili, hoʻonui nui ʻia kona hiki ke hoʻopaʻa ʻia i ka protein, hiki i 300 ~ 400ng IgG/cm2, a ʻo ke kaumaha mole o ka protein paʻa nui he> 10kD.
✦E hoʻokaʻawale ma ke ʻano o lalo
lalo palahalaha:haʻahaʻa refractive index, kūpono no ka ʻike ʻana me nā mea heluhelu microplate;
U lalo:He kiʻekiʻe ka refractive index, kahi kūpono no ka hoʻohui, aspirating, hui ʻana a me nā hana ʻē aʻe.Hiki iā ʻoe ke nānā pono i ka hoʻololi ʻana o ka waihoʻoluʻu ma o ka nānā ʻana me ka ʻole o ka kau ʻana ma luna o ka microplate heluhelu e hoʻoholo ai inā loaʻa kahi hopena kūlohelohe.
Ka manawa hoʻouna: Dec-22-2023